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Enzymatic properties
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Tomilova J.E., Kuznetsov V.V., Abdurashitov M.A., Netesova N.A., Degtyarev S.Kh. Recombinant DNA-methyltransferase M1.Bst19I from Bacillus stearothermophilus 19: purification, properties and amino acids sequence analysis.// Molecular Biology(Moscow), Vol.44, No. 4, 688-698 (2010). (In Russian)
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Chernukhin V.A., Kuznetsov V.V., Gonchar D.A., Kashirina Yu.G., Netesova N.A., Degtyarev S. Kh. Analysis of substrate specificity and biochemical properties of DNA methyltransferase M3.BstF5I from BstF5I restriction-modification system // Biochemistry (Moscow), Vol. 75, No 1, p.78-87 (2010). (In Russian)
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Dedkov V.S. Analysis of Specificity of DNA-Methyltransferase M.AspS9I in Cell Lysate by means of Restriction Endonucleases Blocking. // Biotekhnologia (Moscow), No 4, p.30-39 (2009). (In Russian)
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Dedkov V.S. Determination of Specificity of DNA Methyltransferase M.Bsc4I in Cellular Lysate by Blocking Restriction Endonucleases and Computer Modeling. // Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya (Moscow), No 3, p.3-8 (2009). (In Russian)
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Chernukhin V.A., Seggewiss J., Kashirina Yu.G., Gonchar D.A., Degtyarev S.Kh. Purification of recombinant DNA methyltransferase M2.BstSEI from nickase-modification system NM.BstSEI and study of the enzyme properties. // Molecular Biology(Moscow), Vol.43, No. 1, 10-18 (2009). (In Russian)
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Tarasova G. V., Nayakshina T. N., Degtyarev S. Kh. Substrate specificity of new methyl-directed DNA endonuclease GlaI . // BMC Molecular Biology 2008, 9:7 | | |
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Tomilova J.E., Chernukhin V.A., Degtyarev S.Kh. Dependence of site-specific endonuclease GlaI activity on quantity and location of methylcytosines in the recognition sequence 5`-GCGC-3`. // Bulletin of biotechnology and physico-chemical biology V.2, No 1, p. 30-39 (2006) (In Russian)
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Chernukhin V.A., Kashirina Yu.G., Sukhanova K.S., Abdurashitov M.A., Gonchar D.A., and Degtyarev S. Kh. Isolation and exploration of biochemical propeties of DNA Methyltransferase FauIA modifying Non-palindromic sequence 5'-CCCGC-3'. // Biochemistry (Moscow), Vol. 70, No 6, (2005). (In Russian)
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Akishev A.G., Rechkunova N. I., Tomilova J. E., Lebedeva N. A., Lavrik O. I., and Degtyarev S. Kh. Thermostable DNA polymerase from Thermus thermophilus B35: preparation and study of a modified form of the enzyme with high affinity to ddNTP. // Biochemistry (Moscow), Vol. 68, No 12, (2003). (In Russian)
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Chernukhin V. A., Golikova L. N., Gonchar D. A., Abdurashitov M. A., Kashirina Yu. G., Netesova N. A., and Degtyarev S. Kh. M.BstF5I-2 and M.BstF5I-4 DNA Methyltransferases from BstF5I restriction-modification system of Bacillus stearothermophilus F5. // Biochemistry (Moscow), Vol. 68, No 9, (2003). (In Russian)
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N.A.Netesova, L.N.Golikova, L.G. Ovechkina, A.A. Evdokimov, E.G. Malygin, N.S. Gololobova, D.A. Gonchar and S.Kh.Degtyarev. The restriction-modification system from Bacillus stearothermophilius F5: Comparison study of the DNA methyltransferases M.BstF5I-1 and M.BstF5I-3. // Molecular Biology(Moscow), vol.36, p.1-8 (2002). (In Russian)
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Rechkunova N. I., Okhapkina S. S., Anarbaev R. O., Lokhova I. A., Degtyarev S. Kh., and Lavrik O. I. Thermostable DNA polymerase from Thermus thermophilus B35: Influence of divalent metal ions on the interaction with deoxynucleoside triphosphates. // Biochemistry (Moscow), Vol. 65, No 5 (2000). (In Russian)
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Zakharenko A.L., Khodyreva S.N., Rechkunova N.I., Safronov I.V., Pyshnyi D.V., Degtyarev S.Kh., Lavrik O.I. Photoaffinity labeling of DNA polymerase from Thermus thermophilus and DNA template by photoreactive analogs of dCTP. // Biochemistry (Moscow), Vol. 63, No. 8, 1090-1096 (1998). (In Russian)
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Degtyarev S. Kh., Prikhod'ko E.A., Rechkunova N.I., Prikhod'ko G.G., Krasnykh V.N. Biochemical characterization of Vsp I methyltransferase. // Gene, 157, 65-66 (1995). | | |
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Degtyarev S. Kh., Prikhod'ko E.A., Rechkunova N.I., Gorbunov. Yu. A. Interaction of Vsp I and Tru9 I restriction endonuclease with synthetic oligonucleotides. // Biochimica et Biophysica Acta, 1172, 89-94 (1993). | | |
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Degtyarev S. Kh., Prikhod'ko E.A., Prikhod'ko G.G., Krasnykh V.N. Vsp I methylase belongs to m6A-gamma class of adenine methylases. // Nucleic Acids Research, Vol. 21, No 8., 2015 (1993). | | |
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Lebedev L.R., Pustoshilova N.M., Repin V.E., Degtyarev S.Kh. Isolation of restriction endonuclease RsaI from Rhodopseudomonas sphaeroides and study of its properties. // Applied biochemistry and microbiology(Moscow), Vol.27, No.3, 330-337 (1991). (In Russian)
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Degtyarev S.Kh., Zernov Yu.P. Type II restriction endonucleases: scheme of possible evolution relations between enzymes with palindrome tetra nucleotide recognition sequences. // Molecular Biology(Moscow), Vol.24, No. 5, 1393-1398 (1990). (In Russian)
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