SE-buffers activity and inactivation temperature (20 minutes) Enzyme Recognition sequence Optimal buffer BSA % Activity Optimal temperature, °С Inactivation, °С B G O W Y R Aat II GACGT↑C C↓TGCAG Y - 10 - 25 25 - 50 10 - 25 25 - 50 100 50 37 65 Abs I CC↑TCGAGG GGAGCT↓CC * - 75 - 100 10 - 25 0 - 10 50 - 75 0 - 10 50 37 65 Acc16 I TGC↑GCA ACG↓CGT W - 50 - 75 75 - 100 25 - 50 100 75 - 100 70 37 65 Acc36 I ACCTGC(N)4↑ TGGACG(N)8↓ Y - 25 - 50 25 - 50 50 - 75 50 - 75 100 100 37 65 Acc65 I G↑GTACC CCATG↓G W - 10 - 25 25 - 50 75 - 100 100 10 - 25 100 37 65 AccB1 I G↑GYRCC CCRYG↓G K + 50 - 75 10 - 25 10 - 25 75 - 100 50 - 75 30 37 65 AccB7 I CCANNNN↑NTGG GGTN↓NNNNACC G - 10 - 25 100 25 - 50 50 - 75 50 - 75 100 37 65 AccBS I GAG↑CGG CTC↓GCC Y - 75 - 100 75 - 100 25 - 50 25 - 50 100 100 37 65 Acl I AA↑CGTT TTGC↓AA Y + 0 - 10 0 - 10 0 - 10 0 - 10 100 80 37 65 AclW I GGATC(N)4↑ CCTAG(N)5↓ Y + 75 - 100 50 - 75 0 - 10 0 - 10 100 30 37 65 Aco I Y↑GGCCR RCCGG↓Y G - 50 - 75 100 50 - 75 25 - 50 75 - 100 100 37 65 Acs I R↑AATTY YTTAA↓R W + 25 - 50 50 - 75 50 - 75 100 10 - 25 100 50 80 Acu I CTGAAG(N)16↑ GACTTC(N)14↓ Y+ SAM + 25 - 50 50 - 75 50 - 75 75 - 100 100 50 37 65 Afe I AGC↑GCT TCG↓CGA Y - 10 - 25 25 - 50 75 - 100 75 - 100 100 100 37 65 Ags I TTS↑AA AA↓STT Y + 75 - 100 50 - 75 10 - 25 10 - 25 100 50 37 65 Ahl I A↑CTAGT TGATC↓A B + 100 75 - 100 25 - 50 25 - 50 75 - 100 100 37 N Ajn I ↑CCWGG GGWCC↓ Y - 25 - 50 10 - 25 10 - 25 25 - 50 100 50 55 65 Alu I AG↑CT TC↓GA Y - 75 - 100 75 - 100 10 - 25 50 - 75 100 50 37 65 AluB I AG↑CT TC↓GA B + 100 75 - 100 10 - 25 10 - 25 75 - 100 60 37 65 Ama87 I C↑YCGRG GRGCY↓C W + 10 - 25 50 - 75 75 - 100 100 0 - 10 25 37 65 Apa I GGGCC↑C C↓CCGGG Y + 50 - 75 25 - 50 0 - 10 0 - 10 100 50 37 65 Ars I ↑(N)8GACNNNNNNTTYG(N)11↑ ↓(N)13CTGNNNNNNAARC(N)6↓ Y + 0 - 10 0 - 10 0 - 10 0 - 10 100 20 30 65 AsiG I A↑CCGGT TGGCC↓A O - 10 - 25 25 - 50 100 75 - 100 10 - 25 75 37 65 AspA2 I C↑CTAGG GGATC↓C W + 10 - 25 50 - 75 75 - 100 100 75 - 100 10 37 80 AspLE I GCG↑C C↓GCG O - 10 - 25 75 - 100 100 50 - 75 25 - 50 100 37 80 AspS9 I G↑GNCC CCNG↓G W - 50 - 75 50 - 75 75 - 100 100 50 - 75 75 37 65 AsuC2 I CC↑SGG GGS↓CC Y - 75 - 100 50 - 75 10 - 25 25 - 50 100 40 37 65 AsuHP I GGTGA(N)8↑ CCACT(N)7↓ O - 10 - 25 50 - 75 100 75 - 100 25 - 50 100 37 65 AsuNH I G↑CTAGC CGATC↓G Y + 75 - 100 50 - 75 0 - 10 0 - 10 100 25 37 65 BamH I G↑GATCC CCTAG↓G G + 25 - 50 100 75 - 100 75 - 100 25 - 50 100 37 65 Bar I ↑(N)7GAAGNNNNNNTAC(N)12↑ ↓(N)12CTTCNNNNNNATG(N)7↓ * - 0 - 10 0 - 10 25 - 50 50 - 75 10 - 25 40 37 65 Bbv12 I GWGCW↑C C↓WCGWG O - 0 - 10 10 - 25 100 75 - 100 10 - 25 60 37 80 Bgl I GCCNNNN↑NGGC CGGN↓NNNNCCG 2W - 50 - 75 50 - 75 0 - 10 75 - 100 25 - 50 100 37 65 Bgl II A↑GATCT TCTAG↓A O - 0 - 10 10 - 25 100 25 - 50 10 - 25 100 37 80 Bme18 I G↑GWCC CCWG↓G O - 10 - 25 25 - 50 100 75 - 100 10 - 25 80 37 65 Bmt I GCTAG↑C C↓GATCG W - 10 - 25 50 - 75 50 - 75 100 75 - 100 100 37 65 Bmu I ACTGGG(N)5↑ TGACCC(N)4↓ Y - 75 - 100 75 - 100 25 - 50 10 - 25 100 25 37 65 BpmI CTGGAG(N)16↑ GACCTC(N)14↓ W + 25 - 50 50 - 75 75 - 100 100 50 - 75 100 37 65 Bpu10I CC↑TNAGC GGANT↓CG K - 10 - 25 25 - 50 50 - 75 50 - 75 25 - 50 100 37 80 Bpu14 I TT↑CGAA AAGC↓TT G - 50 - 75 100 25 - 50 25 - 50 75 - 100 100 37 65 Bsa29 I AT↑CGAT TAGC↓TA G + 25 - 50 100 50 - 75 50 - 75 75 - 100 100 37 65 Bsc4 I CCNNNNN↑NNGG GGNN↓NNNNNCC W + 75 - 100 75 - 100 50 - 75 100 25 - 50 90 55 80 Bse1 I ACTGGN↑ TGAC↓CN Y - 75 - 100 75 - 100 25 - 50 10 - 25 100 100 65 80 Bse118 I R↑CCGGY YGGCC↓R O - 0 - 10 50 - 75 100 75 - 100 25 - 50 100 65 80 Bse21 I CC↑TNAGG GGANT↓CC Y - 50 - 75 50 - 75 10 - 25 25 - 50 100 40 37 80 Bse3D I GCAATGNN↑ CGTTAC↓NN G - 10 - 25 100 25 - 50 50 - 75 75 - 100 100 60 80 Bse8 I GATNN↑NNATC CTANN↓NNTAG G - 25 - 50 100 75 - 100 75 - 100 50 - 75 100 60 80 BseP I G↑CGCGC CGCGC↓G G - 50 - 75 100 75 - 100 50 - 75 50 - 75 100 50 65 BseX3 I C↑GGCCG GCCGG↓C O - 10 - 25 25 - 50 100 50 - 75 10 - 25 25 50 80 BslF I GGGAC(N)10↑ CCCTG(N)14↓ Y + 25 - 50 25 - 50 10 - 25 25 - 50 100 50 37 80 Bso31 I GGTCTC(N)1↑ CCAGAG(N)5↓ O + 25 - 50 75 - 100 100 75 - 100 25 - 50 40 55 80 Bsp13 I T↑CCGGA AGGCC↓T 2K - 25 - 50 50 - 75 75 - 100 50 - 75 0 - 10 5 50 65 Bsp1720 I GC↑TNAGC CGANT↓CG G - 50 - 75 100 50 - 75 50 - 75 75 - 100 75 37 80 Bsp19 I C↑CATGG GGTAC↓C 2W + 0 - 10 10 - 25 50 - 75 75 - 100 10 - 25 5 37 65 BspAC I C↑CGC GGC↓G O + 10 - 25 25 - 50 100 75 - 100 10 - 25 100 37 65 BspFN I CG↑CG GC↓GC Y - 50 - 75 75 - 100 75 - 100 50 - 75 100 100 37 65 BssEC I C↑CNNGG GGNNC↓C Y - 50 - 75 50 - 75 50 - 75 75 - 100 100 100 60 80 BssNA I GTA↑TAC CAT↓ATG W + 50 - 75 50 - 75 75 - 100 100 75 - 100 100 37 N BssT1 I C↑CWWGG GGWWC↓C 2K - 10 - 25 25 - 50 25 - 50 75 - 100 10 - 25 100 60 80 Bst2B I C↑TCGTG GAGCA↓C Y + 75 - 100 25 - 50 10 - 25 25 - 50 100 100 60 80 Bst2U I CC↑WGG GGW↓CC G + 75 - 100 100 50 - 75 50 - 75 10 - 25 50 60 80 Bst4C I ACN↑GT TG↓NCA Y - 75 - 100 75 - 100 10 - 25 25 - 50 100 50 65 80 Bst6 I CTCTTC(N)1↑ GAGAAG(N)4↓ Y + 75 - 100 75 - 100 50 - 75 75 - 100 100 100 65 80 BstAC I GR↑CGYC CYGC↓RG W - 75 - 100 75 - 100 50 - 75 100 75 - 100 100 37 80 BstAF I C↑TTAAG GAATT↓C W + 10 - 25 25 - 50 75 - 100 100 25 - 50 100 55 80 BstAP I GCANNNN↑NTGC CGTN↓NNNNACG W - 25 - 50 25 - 50 75 - 100 100 25 - 50 100 60 80 BstAU I T↑GTACA ACATG↓T W - 10 - 25 50 - 75 25 - 50 100 25 - 50 100 37 80 BstBA I YAC↑GTR RTG↓CAY W + 25 - 50 25 - 50 75 - 100 100 25 - 50 50 65 80 BstC8I GCN↑NGC CGN↓NCG Y - 10 - 25 25 - 50 50 - 75 75 - 100 100 100 55 80 BstDE I C↑TNAG GANT↓C G - 75 - 100 100 25 - 50 50 - 75 10 - 25 100 60 80 BstDS I C↑CRYGG GGYRC↓C Y - 0 - 10 75 - 100 50 - 75 25 - 50 100 100 65 80 BstEN I CCTNN↑NNNAGG GGANNN↓NNTCC Y - 50 - 75 50 - 75 25 - 50 25 - 50 100 100 65 80 BstF5 I GGATGNN↑ CCTAC↓NN Y - 75 - 100 50 - 75 25 - 50 50 - 75 100 100 65 80 BstFN I CG↑CG GC↓GC Y - 75 - 100 50 - 75 25 - 50 25 - 50 100 75 60 80 BstH2 I RGCGC↑Y Y↓CGCGR Y + 50 - 75 50 - 75 0 - 10 10 - 25 100 100 65 80 BstHH I GCG↑C C↓GCG Y + 75 - 100 50 - 75 25 - 50 50 - 75 100 100 50 N BstKTI GAT↑C C↓TAG W - 25 - 50 50 - 75 75 - 100 100 50 - 75 100 37 65 BstMA I GTCTC(N)1↑ CAGAG(N)5↓ W + 25 - 50 50 - 75 50 - 75 100 75 - 100 100 55 65 BstMB I ↑GATC CTAG↓ O - 10 - 25 25 - 50 100 75 - 100 10 - 25 100 65 80 BstMC I CGRY↑CG GC↓YRGC B + 100 75 - 100 10 - 25 10 - 25 50 - 75 100 50 80 BstMW I GCNNNNN↑NNGC CGNN↓NNNNNCG Y - 10 - 25 25 - 50 25 - 50 50 - 75 100 40 55 80 BstNS I RCATG↑Y Y↓GTACR B + 100 50 - 75 10 - 25 10 - 25 75 - 100 50 37 65 BstPA I GACNN↑NNGTC CTGNN↓NNCAG Y - 50 - 75 25 - 50 50 - 75 50 - 75 100 100 65 N BstSCI ↑CCNGG GGNCC↓ Y - 50 - 75 50 - 75 50 - 75 50 - 75 100 60 55 80 BstSF I C↑TRYAG GAYRT↓C O + 75 - 100 25 - 50 100 50 - 75 50 - 75 100 60 N BstSL I GKGCM↑C C↓MCGKG G + 50 - 75 100 50 - 75 75 - 100 75 - 100 100 55 65 BstSN I TAC↑GTA ATG↓CAT B - 100 50 - 75 0 - 10 10 - 25 50 - 75 50 37 80 BstV1I GCAGC(N)8↑ CGTCG(N)12↓ G - 75 - 100 100 75 - 100 75 - 100 75 - 100 100 55 80 BstV2 I GAAGAC(N)2↑ CTTCTG(N)6↓ Y + 75 - 100 75 - 100 25 - 50 25 - 50 100 70 55 65 BstX I CCANNNNN↑NTGG GGTN↓NNNNNACC O - 10 - 25 10 - 25 100 75 - 100 25 - 50 100 37 65 BstX2 I R↑GATCY YCTAG↓R G - 75 - 100 100 0 - 10 10 - 25 25 - 50 100 60 80 Bsu I GTATCC(N)6↑ CATAGG(N)5↓ Y - 75 - 100 50 - 75 10 - 25 25 - 50 100 10 37 65 BsuR I GG↑CC CC↓GG G - 75 - 100 100 25 - 50 50 - 75 50 - 75 100 37 80 Btr I CAC↑GTC GTG↓CAG O + 75 - 100 75 - 100 100 75 - 100 75 - 100 100 60 80 Cci I T↑CATGA AGTAC↓T W + 0 - 10 10 - 25 25 - 50 100 75 - 100 100 55 80 CciN I GC↑GGCCGC CGCCGG↓CG Y - 25 - 50 50 - 75 75 - 100 75 - 100 100 100 37 65 Dra I TTT↑AAA AAA↓TTT G + 75 - 100 100 25 - 50 75 - 100 75 - 100 100 37 65 DraIII CACNNN↑GTG GTG↓NNNCAC 2K + 25 - 50 50 - 75 75 - 100 75 - 100 50 - 75 100 37 65 Dri I GACNNN↑NNGTC CTGNN↓NNNCAG Y - 75 - 100 75 - 100 10 - 25 10 - 25 100 40 37 65 DseD I GACNNNN↑NNGTC CTGNN↓NNNNCAG Y + 75 - 100 75 - 100 25 - 50 50 - 75 100 30 37 80 EcoICR I GAG↑CTC CTC↓GAG G + 75 - 100 100 0 - 10 0 - 10 75 - 100 5 37 65 EcoR I G↑AATTC CTTAA↓G * + 50 - 75 75 - 100 75 - 100 75 - 100 50 - 75 50 37 65 EcoR V GAT↑ATC CTA↓TAG W + 0 - 10 25 - 50 50 - 75 100 25 - 50 50 37 80 Ege I GGC↑GCC CCG↓CGG B + 100 75 - 100 10 - 25 50 - 75 75 - 100 100 37 65 Erh I C↑CWWGG GGWWC↓C 2W + 10 - 25 25 - 50 75 - 100 75 - 100 10 - 25 100 37 65 FaeI CATG↑ ↓GTAC Y + 25 - 50 50 - 75 10 - 25 10 - 25 100 100 37 65 FaiI YA↑TR RT↓AY B - 100 50 - 75 10 - 25 25 - 50 25 - 50 100 50 80 FalI ↑(N)8AAGNNNNNCTT(N)13↑ ↓(N)13TTCNNNNNGAA(N)8↓ W+ SAM - 0 - 10 25 - 50 75 - 100 100 50 - 75 70 37 65 FatI ↑CATG GTAC↓ G - 10 - 25 100 25 - 50 10 - 25 50 - 75 100 55 65 Fau I CCCGC(N)4↑ GGGCG(N)6↓ B - 100 25 - 50 0 - 10 0 - 10 50 - 75 50 55 65 FauND I CA↑TATG GTAT↓AC Y + 50 - 75 75 - 100 10 - 25 50 - 75 100 100 37 65 Fbl I GT↑MKAC CAKM↓TG Y - 50 - 75 75 - 100 0 - 10 50 - 75 100 100 55 80 Fok I GGATG(N)9↑ CCTAC(N)13↓ Y - 50 - 75 50 - 75 25 - 50 25 - 50 100 100 37 65 FriO I GRGCY↑C C↓YCGRG Y + 75 - 100 75 - 100 10 - 25 0 - 10 100 25 37 65 Fsp4H I GC↑NGC CGN↓CG Y - 50 - 75 75 - 100 10 - 25 25 - 50 100 100 37 65 Gsa I CCCAG↑C G↓GGTCG W + 10 - 25 25 - 50 75 - 100 100 75 - 100 100 70 N Hae III GG↑CC CC↓GG G - 75 - 100 100 25 - 50 50 - 75 50 - 75 100 37 80 Hga I GACGC(N)5↑ CTGCG(N)10↓ B - 100 75 - 100 10 - 25 25 - 50 50 - 75 50 37 65 Hind II GTY↑RAC CAR↓YTG G + 75 - 100 100 25 - 50 25 - 50 75 - 100 5 37 65 Hind III A↑AGCTT TTCGA↓A W + 10 - 25 25 - 50 0 - 10 100 0 - 10 100 37 80 Hinf I G↑ANTC CTNA↓G O - 25 - 50 75 - 100 100 75 - 100 75 - 100 100 37 80 Hpa I GTT↑AAC CAA↓TTG Y - 0 - 10 50 - 75 10 - 25 25 - 50 100 25 37 65 Hpa II C↑CGG GGC↓C B - 100 50 - 75 10 - 25 25 - 50 50 - 75 50 37 80 HpySE526 I A↑CGT TGC↓A Y - 75 - 100 75 - 100 10 - 25 25 - 50 100 50 37 65 HspA I G↑CGC CGC↓G Y - 50 - 75 50 - 75 25 - 50 25 - 50 100 100 37 80 Kpn I GGTAC↑C C↓CATGG B + 100 25 - 50 25 - 50 25 - 50 75 - 100 50 37 80 Ksp22 I T↑GATCA ACTAG↓T 2K + 50 - 75 75 - 100 50 - 75 50 - 75 25 - 50 100 37 65 Kzo9 I ↑GATC CTAG↓ G - 50 - 75 100 50 - 75 50 - 75 50 - 75 100 37 65 Mab I A↑CCWGGT TGGWCC↓A W + 25 - 50 50 - 75 75 - 100 100 50 - 75 100 37 65 Mbo II GAAGA(N)8↑ CTTCT(N)7↓ Y - 75 - 100 75 - 100 25 - 50 50 - 75 100 50 37 65 Mhl I GDGCH↑C C↓HCGDG W - 10 - 25 25 - 50 75 - 100 100 10 - 25 100 37 80 Mlu I A↑CGCGT TGCGC↓A O - 0 - 10 10 - 25 100 25 - 50 10 - 25 50 37 65 Mly113 I GG↑CGCC CCGC↓GG B - 100 25 - 50 10 - 25 10 - 25 50 - 75 100 37 65 Mnl I CCTC(N)7↑ GGAG(N)6↓ G + 75 - 100 100 25 - 50 25 - 50 75 - 100 100 37 65 MroN I G↑CCGGC CGGCC↓G B - 100 50 - 75 10 - 25 0 - 10 10 - 25 5 37 80 MroX I GAANN↑NNTTC CTTNN↓NNAAG W - 50 - 75 50 - 75 50 - 75 100 25 - 50 100 37 65 Msp I C↑CGG GGC↓C B - 100 75 - 100 50 - 75 75 - 100 75 - 100 100 37 65 Msp20 I TGG↑CCA ACC↓GGT W + 50 - 75 50 - 75 25 - 50 100 50 - 75 80 37 65 MspA1 I CMG↑CKG GKC↓GMC Y - 10 - 25 75 - 100 10 - 25 25 - 50 100 100 37 65 MspR9 I CC↑NGG GGN↓CC O - 50 - 75 50 - 75 100 50 - 75 75 - 100 100 37 80 Nru I TCG↑CGA AGC↓GCT W - 0 - 10 10 - 25 75 - 100 100 10 - 25 100 37 80 PalA I GG↑CGCGCC CCGCGC↓GG Y - 25 - 50 10 - 25 0 - 10 0 - 10 100 40 37 65 Pce I AGG↑CCT TCC↓GGA Y - 75 - 100 75 - 100 50 - 75 25 - 50 100 100 50 80 Pci I A↑CATGT TGTAC↓A O - 50 - 75 75 - 100 100 75 - 100 50 - 75 50 37 65 PciS I GCTCTTCN↑ CGAGAAG(N)4↓ B - 100 50 - 75 0 - 10 0 - 10 75 - 100 50 37 65 Pct I GAATGCN↑ CTTAC↓GN O - 25 - 50 50 - 75 100 75 - 100 10 - 25 50 37 65 Ple19 I CGAT↑CG GC↓TAGC Y - 75 - 100 75 - 100 25 - 50 25 - 50 100 100 37 65 Pps I GAGTC(N)4↑ CTCAG(N)5↓ Y + 50 - 75 10 - 25 0 - 10 25 - 50 100 50 37 65 Psi I TTA↑TAA AAT↓ATT B - 100 25 - 50 10 - 25 25 - 50 75 - 100 40 37 65 Psp124B I GAGCT↑C C↓TCGAG G - 75 - 100 100 10 - 25 0 - 10 75 - 100 30 37 80 Psp6 I ↑CCWGG GGWCC↓ B - 100 50 - 75 10 - 25 25 - 50 75 - 100 10 55 80 PspC I CAC↑GTG GTG↓CAC B + 100 50 - 75 0 - 10 0 - 10 50 - 75 5 37 65 PspE I G↑GTNACC CCANTG↓G B - 100 50 - 75 25 - 50 50 - 75 50 - 75 100 37 65 PspL I C↑GTACG GCATG↓C Y + 75 - 100 75 - 100 25 - 50 10 - 25 100 100 37 65 PspN4 I GGN↑NCC CCN↓NGG Y - 10 - 25 10 - 25 10 - 25 25 - 50 100 50 37 65 PspOM I G↑GGCCC CCCGG↓G Y - 75 - 100 10 - 25 0 - 10 0 - 10 100 25 37 65 PspPP I RG↑GWCCY YCCWG↓GR Y - 10 - 25 10 - 25 0 - 10 0 - 10 100 100 37 65 PspX I VC↑TCGAGB BGAGCT↓CV Y + 50 - 75 50 - 75 25 - 50 75 - 100 100 25 37 80 Psr I ↑(N)7GAACNNNNNNTAC(N)12↑ ↓(N)12CTTGNNNNNNATG(N)7↓ Y + 10 - 25 10 - 25 0 - 10 0 - 10 100 30 30 65 Pst I CTGCA↑G G↓ACGTC O + 10 - 25 25 - 50 100 25 - 50 25 - 50 50 37 80 PstN I CAGNNN↑CTG GTC↓NNNGAC Y - 25 - 50 50 - 75 10 - 25 25 - 50 100 100 37 65 Pvu II CAG↑CTG GTC↓GAC G + 25 - 50 100 25 - 50 25 - 50 25 - 50 100 37 80 Rga I GCGAT↑CGC CGC↓TAGCG Y - 75 - 100 50 - 75 10 - 25 25 - 50 100 100 55 80 Rig I GGCCGG↑CC CC↓GGCCGG * + 75 - 100 50 - 75 0 - 10 10 - 25 50 - 75 10 37 65 Rsa I GT↑AC CA↓TG B - 100 50 - 75 0 - 10 50 - 75 75 - 100 50 37 80 RsaN I G↑TAC CAT↓G B - 100 75 - 100 50 - 75 50 - 75 75 - 100 100 37 80 Rsr2 I CG↑GWCCG GCCWG↓GC Y + 50 - 75 75 - 100 0 - 10 10 - 25 100 25 37 65 Sal I G↑TCGAC CAGCT↓G O - 0 - 10 10 - 25 100 25 - 50 0 - 10 5 37 65 Sbf I CCTGCA↑GG GG↓ACGTCC Y - 75 - 100 50 - 75 0 - 10 0 - 10 100 50 37 80 Set I ASST↑ ↓TSSA Y - 25 - 50 25 - 50 75 - 100 75 - 100 100 100 55 80 SfaN I GCATC(N)5↑ CGTAG(N)9↓ O - 10 - 25 25 - 50 100 75 - 100 0 - 10 25 37 80 Sfi I GGCCNNNN↑NGGCC CCGGN↓NNNNCCGG G + 75 - 100 100 25 - 50 25 - 50 25 - 50 75 50 65 Sfr274 I C↑TCGAG GAGCT↓C B - 100 75 - 100 50 - 75 50 - 75 75 - 100 100 50 65 Sfr303 I CCGC↑GG GG↓CGCC B - 100 50 - 75 10 - 25 10 - 25 75 - 100 100 37 65 Sma I CCC↑GGG GGG↓CCC Y - 0 - 10 0 - 10 0 - 10 0 - 10 100 50 25 65 Smi I ATTT↑AAAT TAAA↓TTTA O + 25 - 50 25 - 50 100 75 - 100 25 - 50 10 37 65 SmiM I CAYNN↑NNRTG GTRNN↓NNYAC W - 10 - 25 10 - 25 75 - 100 100 10 - 25 60 37 65 Sph I GCATG↑C C↓GTACG G + 25 - 50 100 75 - 100 75 - 100 50 - 75 100 37 65 Sse9 I ↑AATT TTAA↓ B + 100 75 - 100 50 - 75 50 - 75 75 - 100 75 55 65 Ssp I AAT↑ATT TTA↓TAA K + 75 - 100 50 - 75 25 - 50 50 - 75 75 - 100 100 37 65 Taq I T↑CGA AGC↓T Y + 50 - 75 75 - 100 75 - 100 50 - 75 100 100 65 80 Tru9 I T↑TAA AAT↓T W - 75 - 100 25 - 50 25 - 50 100 50 - 75 100 65 80 Tth111 I GACN↑NNGTC CTGNN↓NCAG Y - 75 - 100 50 - 75 10 - 25 10 - 25 100 100 65 80 Vne I G↑TGCAC CACGT↓G O - 10 - 25 25 - 50 100 25 - 50 25 - 50 100 37 65 Vsp I AT↑TAAT TAAT↓TA W - 0 - 10 10 - 25 50 - 75 100 25 - 50 50 37 65 Xba I T↑CTAGA AGATC↓T O + 75 - 100 75 - 100 100 50 - 75 75 - 100 25 37 65 Xma I C↑CCGGG GGGCC↓C Y - 75 - 100 50 - 75 0 - 10 0 - 10 100 50 37 65 ZraI GAC↑GTC CTG↓CAG B - 100 50 - 75 25 - 50 25 - 50 75 - 100 100 37 80 Zrm I AGT↑ACT TCA↓TGA Y + 50 - 75 25 - 50 0 - 10 0 - 10 100 50 37 65 Zsp2 I ATGCA↑T T↓ACGTA B + 100 50 - 75 25 - 50 25 - 50 25 - 50 50 37 65

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