Product info: MspR9 I Name MspR9 I      Cat. # E175 E176 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-20000 10000-20000 Add to basket Recognition site CC↑NGG GGN↓CC Source Micrococcus species R9 Assayed on Lambda DNA (dcm-) Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 50 - 75 50 - 75 100 50 - 75 75 - 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol; Store at -20°C. Ligations After 2-fold MspR9 I overdigestion none of the DNA fragments can be ligated. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity Blocked by overlapping Dcm methylation(CmCWGG): CCAGG and CCTGG. Inactivation 20 minutes under 80oC MSDS: Download MSDS as PDF References: Repin, V.E., Andreeva, I.S., Kileva, E.V., Shevchenko, A.V., Abdurashitov, M.A., Repin, M.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 33: 284-286 (1997).