Product info: Msp20 I Name Msp20 I    Cat. # E301 E302 Package, u.a. 100 500 Concentration, u.a./ml 1000-3000 1000-3000 Add to basket Recognition site TGG↑CCA ACC↓GGT Source Micrococcus species 20 Assayed on Lanbda DNA (dcm-) Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y R 50 - 75 50 - 75 25 - 50 100 50 - 75 80 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 2-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete ligation can be reached by using 10% PEG Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA Methylation sensitivity Cleaved of DNA is impaired by overlapping Dcm methylation(CmCWGG): TGGCCAGG. Inactivation 20 minutes under 65oC Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. MSDS: Download MSDS as PDF References: Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K. Russian Patent Office (1993). SU 1806191 A3  Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322