Product info: Kzo9 I Name Kzo9 I        Cat. # E187 E188 Package, u.a. 200 1000 Concentration, u.a./ml 1000-5000 1000-5000 Add to basket Recognition site ↑GATC CTAG↓ Source Kurthia zopfii 9 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 50 - 75 100 50 - 75 50 - 75 50 - 75 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C. Ligations After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity Not blocked by overlapping Dam methylation (GmATC): GATC. Blocked by overlapping CG methylation: CGATmCG. Cleaved of DNA is impaired by overlapping CG methylation: GATmCG. Inactivation 20 minutes under 65oC MSDS: Download MSDS as PDF References: Degtyarev, S.Kh., Rechkunova, N.I., Grinev, A.A., Dedkov, V,S., Izv.Sib.Otd.Akad.Nauk SSSR 15: 25-26 (1989).  M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006  V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322