Product info: Hinf I Name Hinf I      Cat. # E075 E076 E076X Package, u.a. 2000 10000 10000 Concentration, u.a./ml 20000 20000 40000 Add to basket Recognition site G↑ANTC CTNA↓G Source E.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 25 - 50 75 - 100 100 75 - 100 75 - 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity not tested Inactivation 20 minutes under 80oC MSDS: Download MSDS as PDF References: Hutchison, C.A., Barrell, B.G. Unpublished observations. (1992)  Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305  Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007