Product info: FauND I Name FauND I      Cat. # E009 E010 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-20000 10000-20000 Add to basket Recognition site CA↑TATG GTAT↓AC Source E.coli strain that carries the cloned FauND I gene from Flavobacterium aquatili ND Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y R 50 - 75 75 - 100 10 - 25 50 - 75 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1mM DTT; 200 μg/ml BSA, 50% glycerol; Store at -20°C. Ligations After 10-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA Methylation sensitivity not tested Inactivation 20 minutes under 65oC Notes Sensitive to impurities present in some DNA preparations. For example, DNA purified by standard miniprep procedures is cleaved at lowe rates. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. MSDS: Download MSDS as PDF References: Shevchenko, A.V., Belichenko, O.A., Myakisheva, T.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).  Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322