Product info: FaiI


Name
FaiI
Cat. #E551E552
Package, u.a.100500
Concentration, u.a./ml20002000

Recognition site
YATR
RTAY
SourceFlavobacterium aquatile B15
Assayed onDouble-stranded oligonucleotide
5`- CGAGTTCA^TAGCTGGGCCCAAC -3`
3`- GCTCAAGT^ATCGACCCGGGTTG -5`
DescriptionNote! FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity.
In the case of long incubation with FaiI DNA can be digested to small oligos.
Unit definitionOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10050 - 7510 - 2525 - 5025 - 50100
Optimal temperature
50oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.
Reagents Supplied with Enzyme 10 X SE-buffer B
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References: Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press