| Recognition site | YA↑TR
RT↓AY |
| Source | Flavobacterium aquatile B15 |
| Assayed on | Double-stranded oligonucleotide
5’- CGAGTTCA^TAGCTGGGCCCAAC -3’
3’- GCTCAAGT^ATCGACCCGGGTTG -5’ |
| Description | FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity. |
| Unit | One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 50°C in a total reaction volume of
20 μl. |
| Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | | 100 | 50 - 75 | 10 - 25 | 25 - 50 | 25 - 50 |
|
| Optimal temperature | 50oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Ligations | After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut. |
| Non-specific hydrolisis | Fai I overnight digestion greatly enhances the star activity. DNA can be digested to small oligos under "star" conditions.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer B |
| Inactivation 20 minutes under | 80oC |
| MSDS: | Download MSDS as PDF |
| References: |  Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press
|
