| Product info: dNTP Mix, 2.5 μmol of each, water solution, 1ml |
| Name | dNTP Mix, 2.5 μmol of each, water solution, 1ml | | Cat. # | N026 | | Package, μmol | 10 | | Concentration, mmol/l | 10 |
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| Source | Method of production: enzymatic synthesis. | | Description | Deoxynucleotide solution mix is an equimolar solution of ultrapure dATP, dCTP, dGTP
and dTTP, each at a final concentration of 2,5 mM. | | Application | For use in molecular biology and biotechnology, including PCR, long-PCR, and for labeling and sequencing DNA. PCR-diagnostics for medicine. | | Storage conditions | Store at -20°C | | Quality control | Test for detection of endo- and exo-deoxyribonuclease and phosphatase contaminants: No detectable degradation of single-stranded and double-stranded oligonucleotide was observed after incubation of dNTP mix for 3 hour at 37 °C.
Test for detection of RNase contaminants: No detectable degradation of fluorescent-marked oligonucleotide was observed after incubation of dNTP mix for 30 minunts at 37 °C.
Functional test: The product has been
tested for receiving products with
length more than 15,000 base pair
nucleotides with T7 phage DNA
as a template. | | MSDS: | Download MSDS as PDF |
PCR amplification of 15.1 kb DNA fragment. Template – T7 bacteriophage DNA.
Primers:
5 ctcgcaagtctcgccgta 3`
5` cgcattgtctcgccttca 3`
Termocycler profile:
94°C – 2 min
80°C – pause, adding 1 unit TaqSE DNA Polymerase
35 cycles:
94°C – 5 sec
56°C – 10 sec
68°C – 20 min
1% TAE agarose gel
Lane 1 chemically synthesized dNTPs
Lane 2 enzyme-manufactured dNTPs
Lane 3 λ DNA BglI Digest |  |
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