| Recognition site | CG↑CG
GC↓GC |
| Source | Bacillus stearothermophilus FN |
| Assayed on | Lambda DNA |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | | 50 - 75 | 75 - 100 | 75 - 100 | 50 - 75 | 100 |
|
| Optimal temperature | 37oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Methylation sensitivity | Blocked by CG methylation |
| Inactivation 20 minutes under | 65oC |
| MSDS: | Download MSDS as PDF |
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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