| Recognition site | AG↑CT
TC↓GA |
| Source | Arthrobacter luteus B |
| Assayed on | Lambda DNA |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 100 | 75 - 100 | 10 - 25 | 10 - 25 | 75 - 100 | 60 |
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| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme 80% of the Lambda DNA fragments can be ligated with T4 DNA Ligase and can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer B, BSA |
| Methylation sensitivity | AluB I is able to cleave some methylated DNA substrates.
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| Inactivation 20 minutes under | 65oC |
| Notes | AluB I is an isoschizomer of Alu I.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation. |
| MSDS: | Download MSDS as PDF |
| References: |  V.A. Chernukhin, A. A. Boltengagen, G. V. Tarasova, V.S. Dedkov, S.Kh. Degtyarev New Restriction Endonuclease AluBI from Arthrobacter luteus B - AluI Isoshizomer, nonsensitive to Presence of 5-methylcytosine in the Recognition Sequence AGCT // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 1, pp 21-27, 2007
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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