| Source | Isolated from E.coli strain that carries the cloned T7 gene I |
| Description | T7 RNA Polymerase catalyzes the synthesis of RNA in the 5’ -> 3’ direction in the presence of a DNA template containing a T7 phage promoter.
Applications:
-Radiolabeled RNA probe
preparation
-RNA generation for in vitro
translation
-RNA generation for studies of RNA structure,processing and catalysis
|
| Unit | One unit is the amount of enzyme required to incorporate 1 nmol NTP into an acid - insoluble material in 1 hour at 37°C.
|
| Reaction buffer | SE-buffer RNA polymerase T7, (50 mM Tris-HCl (pH 7.5 at 25°C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine.) |
| Optimal temperature | 37oC |
| Storage conditions | 50 mM Tris - HCl (pH 7.5); 100 mM NaCl; 20 mM 2-mercaptoethanol; 1 mM EDTA; 0.1% Triton X-100; 50% glycerol. Store at -20°C. |
| Quality control | Purified free of contaminating endonucleases and exonucleases. |
| MSDS: | Download MSDS as PDF |
