| Name | M3.BstF5 I | | Cat. # | M007 | | Package, u.a. | 1000 | | Concentration, u.a./ml | 10000 |
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| Recognition site | GG(mA)TG
| | Source | E.coli strain, that carries gene M3.BstF5 I from Bacillus stearothermophilus F5. | | Description | M3.BstF5I Methylase modifies the adenin residue (mA) in the recognition secuence 5’-GGATGNN-3’. | | Unit | One unit of the enzyme is the amount required to protect 1 μg
of Lambda DNA in 1 hour at 60°C in a total reaction volume of 20μl against cleavage by BstF5 I restriction endonuclease.
| | Reaction buffer | SE-buffer K + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100mM KCl; 1 mM DTT + 0,08mM SAM.
(32 mM S-adenosylmethionine supplied)) | | Optimal temperature | 60oC | | Storage conditions | 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 50% glycerol. Store at -20°C. | | Notes | To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
| | MSDS: | Download MSDS as PDF | | References: | Chernukhin V.A., Kuznetsov V.V., Gonchar D.A., Kashirina Yu.G., Netesova N.A., Degtyarev S. Kh. Analysis of substrate specificity and biochemical properties of DNA methyltransferase M3.BstF5I from BstF5I restriction-modification system // Biochemistry (Moscow), Vol. 75, No 1, p. 78-87 (2010). (In Russian)
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