| Recognition site | ↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓ |
| Source | Bacillus sphaericus |
| Assayed on | T7 DNA |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | SE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 0 - 10 | 0 - 10 | 25 - 50 | 50 - 75 | 10 - 25 | 40 |
|
| Optimal temperature | 37oC |
| Storage conditions | 20 mM KH2PO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C. |
| Ligations | After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer 2K |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 65oC |
| MSDS: | Download MSDS as PDF |
