| Recognition site | Y↑GGCCR
RCCGG↓Y |
| Source | Acinetobacter calcoaceticus |
| Assayed on | Lambda DNA(dam-, dcm-) |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-, dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 50 - 75 | 100 | 50 - 75 | 25 - 50 | 75 - 100 | 100 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Ligations | After 3-fold overdigestion with AcoI more than 90% of Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of AcoI for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer G |
| Methylation sensitivity | Blocked by overlapping dcm methylation (CmCWGG):
CCTGGCCR.
|
| Inactivation 20 minutes under | 65oC |
| MSDS: | Download MSDS as PDF |
| References: | Chernukhin, V.A., Belichenko, O.A., Tomilova, J.E., Dedkov, V.S., Doroganov, A.O.. Unpublished observations. (2006)
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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