| Name | M.HspA I | | Cat. # | M003 | | Package, u.a. | 100 | | Concentration, u.a./ml | 1000-3000 |
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| Recognition site | G(5mC)GC
| | Source | E.coli strain, that carries gene M.HspAI from Haemophilus species AI. | | Description | M.HspAI Methylase modifies the internal cytosine residue (C5) in the recognition secuence 5’-GCGC-3’. | | Unit | One unit of the enzyme is the amount required to protect 1 μg
of Lambda DNA in 1 hour at 37°C in a total reaction volume of 20μl against cleavage by HspAI restriction endonuclease.
| | Reaction buffer | SE-buffer B + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 0,08 mM SAM.
(32 mM S-adenosylmethionine supplied)) | | Optimal temperature | 37oC | | Storage conditions | 10 mM Tis-HCl (pH 7.4); 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 100 mM NaCl; 50% glycerol. Store at -20°C. | | Notes | To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
| | MSDS: | Download MSDS as PDF |
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