| Name | M.Fsp4H I | | Cat. # | M001 | | Package, u.a. | 100 | | Concentration, u.a./ml | 500-1000 |
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| Recognition site | G(m5C)NGC
| | Source | E.coli strain, that carries gene M.Fsp4HI from Flavobacterium species 4H | | Description | M.Fsp4HI Methylase modifies the internal cytosine residue (C5) in the recognition secuence. | | Unit | One unit of the enzyme is the amount required to protect 1 μg
of Lambda DNA in 1 hour at 30°C in a total reaction volume of 20 μl against cleavage by Fsp4HI restriction endonuclease.
| | Reaction buffer | SE-buffer B + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 0,08 mM SAM.
(32 mM S-adenosylmethionine supplied)) | | Optimal temperature | 30oC | | Storage conditions | 10 mM Tis-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. | | Notes | To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
| | MSDS: | Download MSDS as PDF | | References: | Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh. Gene cloning, comparative analysis of the protein structures from Fsp4HI restriction-modification system and biochemical characterization of the recombinant DNA methyltransferase M.Fsp4HI. // Molecular Biology, V.41, No 1, pp 43-50 (2007) (In Russian)
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