| Recognition site | G(mA)↑TC
CT↓(mA)G |
| Source | Marinococus albus I |
| Substrate specifity | The enzyme cleaves only methylated DNA. |
| Assayed on | pBR322 DNA (dam-methylated) |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Reaction buffer | SE-buffer MalI, (20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C. |
| Ligations | After 2-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer MalI |
| Methylation sensitivity | The enzyme cleaves only methylated DNA. |
| Inactivation 20 minutes under | 65oC |
| MSDS: | Download MSDS as PDF |
