| Source | Isolated from E.coli strain that carries the cloned Taq DNA polymerase gene |
| Description | Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity and a double strand specific 5` -> 3` exonuclease activity.
Applications:
- nick translation;
- primer extension reaction.
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| Unit | One unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Unit Assay Conditions: 1 x Taq-DNA-polymerase buffer, 200 µM dNTPs including [3H]-dTTP and 250 µg/ml activated calf thymus DNA.
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| Reaction buffer | SE-buffer DNA polymerase Taq, (60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.) |
| Optimal temperature | 72oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C. |
| Quality control | DNA free.
Purified free of contaminating endonucleases and exonucleases.
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| Legal |
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. |
| MSDS: | Download MSDS as PDF |
