| Recognition site | GCANNNN↑NTGC
CGTN↓NNNNACG |
| Source | Bacillus stearothermophilus AP |
| Assayed on | Lambda DNA |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 25 - 50 | 25 - 50 | 75 - 100 | 100 | 25 - 50 | 100 |
|
| Optimal temperature | 60oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer W |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 80oC |
| Notes | High enzyme concentration may result in star activity. At 37°C activiti is 50% from maximum.
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5. |
| MSDS: | Download MSDS as PDF |
| References: | Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Nucleic Acids Res. 25: 2301-2302 (1997).
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