| Source | Isolated from E.coli strain that carries the cloned Inorganic pyrophosphatase gene from Thermococcus litoralis. |
| Description | Tli-Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4+H2O -> 2HPO4-2. Enzyme is extremely thermostable. |
| Unit | One unit is the amount of enzyme that will generate 40 nmoles of phosphate per minute from pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tris-HCl (pH 8.5), 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml). |
| Reaction buffer | SE-buffer Inorganic pyrophosphatase, (50 mM Tris-HCl (pH 8.5 at 25°C); 1 mM MgCl2.) |
| Optimal temperature | 75oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.6); 0.1 mM EDTA; 100 mM KCl, 0.2% Tween-20; 50% glycerol. Store at -20°C. |
| Quality control | Free of exonuclease and endonuclease activities. |
| MSDS: | Download MSDS as PDF |
