Product info: RNA ligase T4 Name RNA ligase T4 Cat. # E349 E350 Package, u.a. 1000 5000 Concentration, u.a./ml 10000 10000 Add to basket Source Isolated from E.coli strain that carries the cloned RNA ligase gene from bacteriophage T4 Description RNA Ligase catalyzes ligation of a 5’ phosphoryl - terminated nucleic acid donor to a 3’ hydroxyl - terminated nucleic acid acceptor through the formation of a 3’ -> 5’ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. Applications: - labeling of 3’-termini of RNA with 5’ - [32P] pCp; - inter- and intra-molecular joining of RNA and DNA molecules. Unit One unit is defined as the amoumt of enzyme required to convert of 1 pmol of [3H]ATP in AMP –ligase complex in 15 minutes at 25°C. Unit Assay Conditions: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM DTT, 0.2 µM [3H]ATP. Reaction buffer SE-buffer RNA ligase T4, (50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.) Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C. Quality control Free of contaminating single-stranded DNA exonuclease, endonuclease, ribonuclease and phosphatase activities. MSDS: Download MSDS as PDF