Product info: Exonuclease III Name Exonuclease III    Cat. # E345 E346 Package, u.a. 5000 25000 Concentration, u.a./ml 40000-100000 40000-100000 Add to basket Source Isolated from E.coli. Description Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Applications: - unidirectional nested deletions; - site-directed mutagenesis; - preparation of strand-specific probes; - preparation of single-stranded substrates for dideoxy sequencing. Unit One unit is the amount of enzyme required to produce 1 nmol of acid soluble total nucleotide in 30 minutes at 37°C. Unit Assay Conditions: 50 mM Tris-HCl (pH 7.5 at 25°C); 1 mM MgCl2; 1 mM DTT, 0.15 mM sonicated pancreatic DNA. Reaction buffer SE-buffer Exonuclease III, (50 mM Tris-HCl (pH 7.6 at 25°C); 1 mM MgCl2.) Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. MSDS: Download MSDS as PDF