Product info: Alkaline Phosphatase Name Alkaline Phosphatase Cat. # E327 E328 Package, u.a. 100 500 Concentration, u.a./ml 10000 10000 Add to basket Source Alkaline Phosphatase mucose Description Alkaline phosphatase catalyzes the removal of 5' phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates. Applications: - removing 5’ and 3’ phosphoryl groups from nucleic acids; - preparing templates for 5’ end labeling; - preventing fragments from self ligating; - dephosphorylation of proteins Unit One unit is the amount of enzyme that hydrolyzes 1 µmol of p-nitrophenylphosphate to p- nitrophenol in a total reaction volume of 1 ml in 1 min at 37°C. Unit Assay Conditions: 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl2 and 10 mM p-nitrophenylphosphate. These conditions are only used for quantitating enzyme activity. Reaction buffer SE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Optimal temperature 37oC Storage conditions 50 mM KCl, 10 mM Tris-HCl (pH 8.2), 1 mM MgCl2, 0.1 mM ZnCl2, 50% glycerol. Store at -20°C Quality control Purified free of contaminating exonuclease, endonuclease, ribonuclease activities. MSDS: Download MSDS as PDF