|Product info: Uracil DNA Glycosylase|
|Uracil DNA Glycosylase|| |
|Cat. #||E335||E336 |
|Package, u.a.||1000||5000 |
|Concentration, u.a./ml||20000-50000||20000-50000 |
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|Source||Isolated from the recombinant source|
|Description||E.coli uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases)|
- site-directed mutagenesis;
- as a probe for protein-DNA interaction studies;
Treatment of 1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes.
|Unit definition||One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µl reaction containing 0,2 µg DNA
(104 – 105 cpm/µg) in 30 minutes at 37°C.
|Reaction buffer||SE-buffer Uracil DNA Glycosylase, (20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT)|
|Storage conditions||10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.|
|MSDS:||Download MSDS as PDF|