Product info: M-MuLV Reverse transcriptase, RNase H- Name M-MuLV Reverse transcriptase, RNase H- Cat. # E317 E318 Package, u.a. 5000 25000 Concentration, u.a./ml 100000-500000 100000-500000 Add to basket Source Isolated from E.coli strain that carries the cloned Reverse transcriptase gene Description Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs. Application: first strand cDNA synthesis. Unit One unit is the amount of the enzyme required to incorporate 1 nmol of dTTP into an acid insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT). Unit Assay Conditions: 50 mM Tris-HCl (pH 8.3), 6 mM MgCl2, 10 mM DTT, 0.5 mM [3H]-dTTP, 0.4 mM poly(rA)•oligo(dT) 12-18. Reaction buffer SE-buffer Reverse transcriptase M-MulV, (50 mM Tris-HCl (pH 8.3 at 25°C); 3 mM MgCl2; 75 mM KCl; 10 mM DTT.) Optimal temperature 37oC Storage conditions 10 mM KH2PO4 (pH 7.5); 0,1 mM EDTA; 200 mM NaCl; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. Quality control Purified free of detectable levels of RNase, endonuclease and exonuclease activities. MSDS: Download MSDS as PDF