| Recognition site | ACN↑GT
TG↓NCA |
| Source | Bacillus stearothermophilus 4C |
| Assayed on | Lambda DNA |
| Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 75 - 100 | 75 - 100 | 10 - 25 | 25 - 50 | 100 | 50 |
|
| Optimal temperature | 65oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme less than 10% of DNA fragments can be ligated. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 80oC |
| MSDS: | Download MSDS as PDF |
| References: | Shinkarenko, N.M., Schevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1997).
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