| Source | Isolated from E.coli strain that carries the cloned T4 DNA polymerase gene |
| Description | T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’ -> 3’ direction and requires the presence of template and primer. This enzyme has a 3’ -> 5’ exonuclease activity.
Applications:
- polishing ends;
- probe labeling using replacement synthesis.
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| Unit | One unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid precipitable material in 30 minutes at 37°C.
Unit Assay Conditions: 1 x T4 DNA Polymerase Reaction Buffer, 33 µM dNTPs including [3H]-dTTP and 70 µg/ml denatured calf thymus DNA.
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| Reaction buffer | SE-buffer DNA polymerase T4, (67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;
16.7 mM (NH4)2SO4, 1 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol. Store at -20°C. |
| Quality control | Purified free of contaminating endonucleases and exonucleases. |
| MSDS: | Download MSDS as PDF |
