Product info: BssEC I Name BssEC I    Cat. # E273 E274 Package, u.a. 200 1000 Concentration, u.a./ml 10000 10000 Add to basket Recognition site C↑CNNGG GGNNC↓C Source Bacillus stearothermophilus EC Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y R 50 - 75 50 - 75 50 - 75 75 - 100 100 100 Optimal temperature 60oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity not tested Inactivation 20 minutes under 80oC MSDS: Download MSDS as PDF References: Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1998).  Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305  Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007