Product info: BseP I Name BseP I    Cat. # E181 E182 Package, u.a. 200 1000 Concentration, u.a./ml 5000 5000 Add to basket Recognition site G↑CGCGC CGCGC↓G Source Bacillus stearothermophilus P Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 50 - 75 100 75 - 100 50 - 75 50 - 75 100 Optimal temperature 50oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity Blocked by CG methylation. Inactivation 20 minutes under 65oC MSDS: Download MSDS as PDF References: Langdale, J.A., Myers, P.A., Roberts, R.J. Unpublished observations (1992). Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322