| Recognition site | GCNNNNN↑NNGC
CGNN↓NNNNNCG |
| Source | Bacillus stearothermophilus MW |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 10 - 25 | 25 - 50 | 25 - 50 | 50 - 75 | 100 | 40 |
|
| Optimal temperature | 55oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol; and 50% glycerol.
Store at -20°C.
For long term storage (more than 7 days), store at -70ºC. |
| Ligations | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 80oC |
| Notes | At 37°C activity is 20% from maximum
|
| MSDS: | Download MSDS as PDF |
| References: | Nadeev A.N., Dedkov, V.S., Tomilova J.E., Degtyarev, S.K. Unpublished observations (2002).
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