| Name | | Alu I |  |
| | Cat. # | E015 | E016 | | Package, u.a. | 200 | 1000 | | Concentration, u.a./ml | 2000-5000 | 2000-5000 |
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| Recognition site | AG↑CT
TC↓GA | | Source | E.coli strain that carries the cloned Alu I gene from Arthrobacter luteus | | Assayed on | Lambda DNA | | Unit | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | | Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) | | Enzyme activity (%) | | B | G | O | W | Y | R | | 75 - 100 | 75 - 100 | 10 - 25 | 50 - 75 | 100 | 50 |
| | Optimal temperature | 37oC | | Storage conditions | 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA; 1 mM DTT, 50% glycerol; Store at -20°C. | | Ligations | After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer Y | | Methylation sensitivity | not tested | | Inactivation 20 minutes under | 65oC | | MSDS: | Download MSDS as PDF | | References: | Roberts, R.J., Myers, P.A., Morrison, A., Murray, K. J. Mol.Biol. 102: 157-165 (1976).
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
V.A. Chernukhin, A. A. Boltengagen, G. V. Tarasova, V.S. Dedkov, S.Kh. Degtyarev New Restriction Endonuclease AluBI from Arthrobacter luteus B - AluI Isoshizomer, nonsensitive to Presence of 5-methylcytosine in the Recognition Sequence AGCT // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 1, pp 21-27, 2007
| Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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