| Name | | BstSCI |   |
| | Cat. # | E307 | E308 | | Package, u.a. | 100 | 500 | | Concentration, u.a./ml | 2000-5000 | 2000-5000 |
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| Recognition site | ↑CCNGG
GGNCC↓ | | Source | Bacillus stearothermophilus SC | | Assayed on | Lambda DNA (dcm-) | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl. | | Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) | | Enzyme activity (%) | | B | G | O | W | Y | R | | 50 - 75 | 50 - 75 | 50 - 75 | 50 - 75 | 100 | 60 |
| | Optimal temperature | 55oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C. | | Ligations | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer Y | | Methylation sensitivity | Blocked by Dcm-methylation (CmCWGG) CCWGG. | | Inactivation 20 minutes under | 80oC | | Notes | At 37°C activity is 10% from maximum.
| | MSDS: | Download MSDS as PDF | | References: | Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K.
Unpublished observations (2001).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
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