Product info: Pct I Name Pct I    Cat. # E045 E046 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-40000 10000-40000 Add to basket Recognition site GAATGCN↑ CTTAC↓GN Source Planococcus citreus SM Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 25 - 50 50 - 75 100 75 - 100 10 - 25 50 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; and 50% glycerol. Store at -20°C. Ligations After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity not tested Inactivation 20 minutes under 65oC MSDS: Download MSDS as PDF References: Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).  V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322