Product info: Vne I Name Vne I Cat. # E137 E138 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-20000 10000-20000 Add to basket Recognition site G↑TGCAC CACGT↓G Source E.coli strain, that carries the cloned gene VneI from Vibrio nereis 18 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y R 10 - 25 25 - 50 100 25 - 50 25 - 50 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligations After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity not tested Inactivation 20 minutes under 65oC MSDS: Download MSDS as PDF References: Degtyarev, S.Kh., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 422-423 (1987). Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322