| Source | Isolated from E.coli strain that carries the cloned Alkaline Phosphatase gene from Alteromonas undina P2 |
| Description | Thermolabile Alkaline phosphatase catalyzes the removal of 5' phosphate groups from DNA, RNA and ribo- and
deoxyribonucleoside triphosphates.
|
| Unit | One unit is the amount of enzyme that will dephosphorylate 1 μg of pUC19 DNA (linearized with Hind III) in 30 minutes at 25°C.
Dephosphorylation is defined as >95% inhibition of recirculation in a self-ligation reaction that is measured by transformation into E.coli. |
| Reaction buffer | SE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 25oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.6), 0.1 mM ZnCl2, 50% glycerol. Store at -20°C |
| Inactivation 20 minutes under | 65oC |
| Quality control | Purified free of contaminating exonuclease, endonuclease, ribonuclease activities. |
| MSDS: | Download MSDS as PDF |
