Product info: Acs I Name Acs I Cat. # E013 E014 Package, u.a. 500 2500 Concentration, u.a./ml 10000-20000 10000-20000 Add to basket Recognition site R↑AATTY YTTAA↓R Source Arthrobacter citreus Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl. Optimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y R 25 - 50 50 - 75 50 - 75 100 10 - 25 100 Optimal temperature 50oC Storage conditions 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; glycerol; Store at -20°C. Ligations After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 50 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA Methylation sensitivity not tested Inactivation 20 minutes under 80oC Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. MSDS: Download MSDS as PDF References: Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.I., Abdurashitov, M.A., Nucleic Acids Res. 20: 3789 (1992).  Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322