| Name | | Sse9 I |   |
| | Cat. # | E217 | E218 | | Package, u.a. | 500 | 2500 | | Concentration, u.a./ml | 5000 | 5000 |
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| Recognition site | ↑AATT
TTAA↓ | | Source | E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species | | Assayed on | pBR322 DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. | | Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA | | Enzyme activity (%) | | B | G | O | W | Y | R | | 100 | 75 - 100 | 50 - 75 | 50 - 75 | 75 - 100 | 75 |
| | Optimal temperature | 55oC | | Storage conditions | 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. | | Ligations | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer B, BSA | | Methylation sensitivity | not tested | | Inactivation 20 minutes under | 65oC | | Notes | At 37°C activity is 75% from maximum.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
| | MSDS: | Download MSDS as PDF | | References: | Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
| Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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