Product info: Sfr303 I Name Sfr303 I Cat. # E127 E128 Package, u.a. 1000 5000 Concentration, u.a./ml 5000-20000 5000-20000 Add to basket Recognition site CCGC↑GG GG↓CGCC Source Streptomyces fradiae 303 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Enzyme activity (%) B G O W Y R 100 50 - 75 10 - 25 10 - 25 75 - 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B Methylation sensitivity not tested Inactivation 20 minutes under 65oC MSDS: Download MSDS as PDF References: Puchkova, L.I., Selina, A.V., Serov, G.D., Krivopalova, G.N., Rechkunova, N.I., Degtyarev, S.Kh., Unpublished observations.  Dedkov V.S., Degtyarev S. Kh. The resctriction endonucleases detection in colonies of microorganisms Streptomyces and Nocardia // APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol 28, 1992, #. 2, 309- 313 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322