| Recognition site | G↑GGCCC
CCCGG↓G |
| Source | E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164 |
| Assayed on | Lambda DNA (BamHI-digest) |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (BamHI-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 75 - 100 | 10 - 25 | 0 - 10 | 0 - 10 | 100 | 25 |
|
| Optimal temperature | 37oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 65oC |
| Notes | The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. |
| MSDS: | Download MSDS as PDF |
| References: | Belichenko, O.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh.
Unpublished observations (1994).
|
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
|
