| Recognition site | A↑CATGT
TGTAC↓A |
| Source | E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45 |
| Assayed on | T7 DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | R | | 50 - 75 | 75 - 100 | 100 | 75 - 100 | 50 - 75 | 50 |
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| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer O |
| Methylation sensitivity | Not blocked by ACmATGT methylation.
Blocked by mACATGT methylation.
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| Inactivation 20 minutes under | 65oC |
| Notes | The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. |
| MSDS: | Download MSDS as PDF |
| References: | Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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