| Buffer | Content |
| SE-buffer B | 10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT. |
| SE-buffer G | 10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT. |
| SE-buffer O | 50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT. |
| SE-buffer W | 10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT. |
| SE-buffer Y | 33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT. |
| SE-buffer K | 10 mM Tris-HCl (pH 7.6 at 250C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT. |
| SE-buffer 2K | 10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT. |
| SE-buffer AbsI | 10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT. |
| SE-buffer BisI | 10 mM Tris-HCl (pH 9,0 at 25°C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT. |
| SE-buffer EcoRI | 100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT. |
| SE-buffer GlaI | 10 mM Tris-HCl (pH 8,5 at 25°C); 5 mM MgCl2; 10 mM NaCl; 1 mM 2-mercaptoethanol. |
| SE-buffer GluI | 10 mM Tris-HCl (pH 9,0 at 25°C); 7,5 mM MgCl2; 75 mM NaCl; 1 mM 2-mercaptoethanol. |
| SE-buffer MalI | 20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT. |
| SE-buffer N.Bst9I | 10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT. |
| SE-buffer RigI | 10 mM Tris-HCl (pH 8.5 at 25°C); 5 mM MgCl2; 1mM DTT. |
| SE-buffer polynucleotide kinase T4 | 50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT. |
| SE-buffer DNA ligase T4 | 50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP; 25 μg/ml BSA. |
| SE-buffer Klenow fragment | 50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT. |
| SE-buffer AS (Ammonium Sulphate) | 67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2. |
| SE-buffer DNA polymerase Taq | 60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100. |
| SE-buffer DNA polymerase T4 | 67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;
16.7 mM (NH4)2SO4, 1 mM DTT. |
| SE-buffer RNA polymerase T7 | 50 mM Tris-HCl (pH 7.5 at 25°C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine. |
| SE-buffer Reverse transcriptase M-MuLV | 50 mM Tris-HCl (pH 8.3 at 25°C); 3 mM MgCl2; 75 mM KCl; 10 mM DTT. |
| SE-buffer Inorganic pyrophosphatase | 50 mM Tris-HCl (pH 8.5 at 25°C); 1 mM MgCl2. |
| SE-buffer Exonuclease III | 50 mM Tris-HCl (pH 7.6 at 25°C); 1 mM MgCl2. |
| SE-buffer Uracil DNA Glycosylase | 20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT |
| SE-buffer 2W | 20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2;; 200 mM NaCl; 1 mM DTT |
| SE-buffer Endonuclease I | 20 mM Glycine-NaOH (pH 9.5 at 25°C); 25 mM MgCl2; 100mM NaCl; 1mM 2-mercaptoethanol |
| SE-buffer Alkaline phosphatase | 50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT. |
| SE-buffer RNA ligase T4 | 50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP. |
| SE-buffer Hot Start Taq DNA polymerase | 67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2. |
| SE-buffer Pfu DNA polymerase | 20 mM Tris-HCl (pH 8.8 at 25°C); 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 0.1 % Triton X-100 )+ 100 μg/ml BSA |
| SE-buffer A (Storage and dilution) | 10 mM Tris-HCl (pH 7.6 at 25°C); 50 mM KCl; 0,1 mM EDTA; 200 mkg/ml BSA; 1mM DTT; 50% glycerol. |
